4 μl of 5 × transstart® fastpfu buffer (TransGen biotech co)

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TransGen biotech co 4 μl of 5 × transstart® fastpfu buffer
4 μl Of 5 × Transstart® Fastpfu Buffer, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fastpfu+buffer+(5/fastpfu+buffer/pm39001641-58-34-23
Average 90 stars, based on 1 article reviews

4 μl of 5 × transstart® fastpfu buffer - by Bioz Stars, 2026-11

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Article Title: Gut microbiome as biomarker for triclosan toxicity in Labeo rohita: bioconcentration, immunotoxicity and metagenomic profiling.
Article Snippet: Triclosan (TCS) is a lipophilic, broad spectrum antimicrobial agent commonly used in personal care products with a projected continuous escalation in aquatic environments in the post COVID 19 era.. There is rich documentation in the literature on the alteration of physiological responses in fish due to TCS exposure; however, studies on gut associated bacteria of fish are still scarce.. This is the first attempt to determine changes in bacterial community structure due to exposure of TCS on Labeo rohita, a commercially essential freshwater species, using 16S V3-V4 region ribosomal RNA (rRNA) next-generation sequencing (NGS).

Article Title: Compositional Shifts in the Mycobiota of 'Shine Muscat' Grape ( Vitis labruscana Baily × V. vinifera L.) Bunches During Cold Storage at Different Temperatures.
Article Snippet: .. PCR reactions were conducted in triplicate in a 20 μL mixture containing 4 μL of 5 × FastPfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of each primer (5 μM), 0.4 μL of FastPfu Polymerase (All PCR reagents were purchased from Beijing TransGen Biotech Co., Ltd., Beijing, China), and 10 ng of template DNA on a GeneAmp 9700 thermocycler (ABI, Foster city, CA, USA) with the following program: 3 min of denaturation at 95 ◦C, 33 cycles of 30 s at 95 ◦C, 30 s for annealing at 55 ◦C, and 45 s for elongation at 72 ◦C, and a final extension at 72 ◦C for 10 min. .. The PCR products were then extracted from a 2% agarose gel, purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA), and quantified using QuantiFluorTM-ST (Promega, Madison, WI, USA) according to the manufacturer’s protocol.

Article Title: Compositional Shifts in the Mycobiota of ‘Shine Muscat’ Grape ( Vitis labruscana Baily × V. vinifera L.) Bunches During Cold Storage at Different Temperatures
Article Snippet: .. PCR reactions were conducted in triplicate in a 20 μL mixture containing 4 μL of 5 × FastPfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of each primer (5 μM), 0.4 μL of FastPfu Polymerase (All PCR reagents were purchased from Beijing TransGen Biotech Co., Ltd., Beijing, China), and 10 ng of template DNA on a GeneAmp 9700 thermocycler (ABI, Foster city, CA, USA) with the following program: 3 min of denaturation at 95 °C, 33 cycles of 30 s at 95 °C, 30 s for annealing at 55 °C, and 45 s for elongation at 72 °C, and a final extension at 72 °C for 10 min. .. The PCR products were then extracted from a 2% agarose gel, purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA), and quantified using QuantiFluorTM-ST (Promega, Madison, WI, USA) according to the manufacturer’s protocol.

Article Title: A potential bioaerosol source from kitchen chimneys in restaurants.
Article Snippet: .. The bacterial 16S rRNA gene and fungal ITS region were amplified in triplicate using a 20 μL mixture including 4 μL of 5 × FastPfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of each primer (5 μM), 0.4 μL of FastPfu Polymerase, and 10 ng of template DNA using an ABI GeneAmp® PCR system 9700 (Applied Biosystems, CA, USA). ..

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Article Title: Soil Properties Regulate Soil Microbial Communities During Forest Succession in a Karst Region of Southwest China
Article Snippet: .. The bacterial amplification solution consisted of 4 μL FastPfu Buffer (5×), 2 μL dNTPs (2.5 mM), 0.8 μL each of Forward Primer (5 μM) and Reverse Primer (5 μM), 0.4 μL FastPfu Polymerse (Beijing TransGen Biotech Co., Ltd., Beijing, China), 0.2 μL BSA, 10 ng Template DNA, and ddH 2 O to a final volume of 20 μL. ..

Article Title: Soil Properties Regulate Soil Microbial Communities During Forest Succession in a Karst Region of Southwest China.
Article Snippet: .. The bacterial amplification solution consisted of 4 μL FastPfu Buffer (5×), 2 μL dNTPs (2.5 mM), 0.8 μL each of Forward Primer (5 μM) and Reverse Primer (5 μM), 0.4 μL FastPfu Polymerse (Beijing TransGen Biotech Co., Ltd., Beijing, China), 0.2 μL BSA, 10 ng Template DNA, and ddH2O to a final volume of 20 μL. ..

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4 μl of 5 × transstart® fastpfu buffer (TransGen biotech co)

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